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274 Additionally, using the above model, it was found that palmitic acid (PA) induced ferroptosis through mitochondrial DNA damage activation, resulting in calcification damage of vascular smooth muscle cells

10.1038/nchembio.2239 123 DongJ.ZhouK.GeX.XuN.WangX.HeQ.et al (2022)

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8-hydroxy-2'-deoxyguanosine (8-OHdG), which reflects oxidative DNA damage, shows measurable increases around the same timeframe

Cytotoxicity Cell culture In vitro, the cytotoxicity of silymarin NCs was assessed by employing the MTT test in conjunction with the MDA-MB-231 cell line